Lessons from Escherichia coli genes similarly regulated in response to nitrogen and sulfur limitation

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Lessons from Escherichia coli genes similarly regulated in response to nitrogen and sulfur limitation.

We previously characterized nutrient-specific transcriptional changes in Escherichia coli upon limitation of nitrogen (N) or sulfur (S). These global homeostatic responses presumably minimize the slowing of growth under a particular condition. Here, we characterize responses to slow growth per se that are not nutrient-specific. The latter help to coordinate the slowing of growth, and in the cas...

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Sulfur and nitrogen limitation in Escherichia coli K-12: specific homeostatic responses.

We determined global transcriptional responses of Escherichia coli K-12 to sulfur (S)- or nitrogen (N)-limited growth in adapted batch cultures and cultures subjected to nutrient shifts. Using two limitations helped to distinguish between nutrient-specific changes in mRNA levels and common changes related to the growth rate. Both homeostatic and slow growth responses were amplified upon shifts....

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-regulated genes in Escherichia coli

Accepted 9 March, 2006. *For correspondence. E-mail pjkiley @wisc.edu; Tel. ( + 1) 608 262 6632; Fax ( + 1) 608 262 5253. Present address: † The Burnham Institute, La Jolla, CA 92037, USA; ‡ Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI 53706, USA. IscR-dependent gene expression links iron-sulphur cluster assembly to the control of O 2 -regulated genes in Escherichi...

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Nitrogen regulatory protein C (NtrC) of enteric bacteria activates transcription of genes/operons whose products minimize the slowing of growth under nitrogen-limiting conditions. To reveal the NtrC regulon of Escherichia coli we compared mRNA levels in a mutant strain that overexpresses NtrC-activated genes [glnL(Up)] to those in a strain with an ntrC (glnG) null allele by using DNA microarray...

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Utilizing the bicistronic reporter transposon mini-Tn5 lacZ-tet/1, we have identified lacZ fusions to four Escherichia coli genes/operons that are strongly activated by the accumulation of self-produced extracellular signals. These fusions were designated cma9, cma48, cma113, and cma114 for conditioned medium activated. Each of the cma fusions was expressed in a growth phase-dependent manner, a...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2005

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.0500141102